Octet^® Systems

The Octet platform includes instruments, biosensors, reagents and assay kits for analysis of biomolecular interactions in 96- and 384-well microplates. Octet systems enable real-time, label-free analysis for determination of affinity, kinetics and concentration. Significantly easier, faster and better characterization of drug candidates is possible, providing greater value in drug development applications where existing methods have limitations in throughput, performance and cost.

• Label-Free, Real-Time Results. Our proprietary Bio-Layer Interferometry (BLI) technology monitors the binding of proteins and other biomolecules to their partners directly in real time.
• Higher Throughput. Octet systems perform sample analysis in 96- and 384-well microplates, processing up to 96 samples in parallel. Protein quantitation of 96 samples can be completed in as little as two minutes — a task that can take from 4 hours to overnight using traditional ELISA. Rapid kinetics, screening and epitope binning assays are enabled by the multiplexed biosensor formats.
• High Quality Data at an Affordable Price. Octet systems provide definitive analysis of kinetic binding constants at a fraction of the price of traditional SPR systems.
• Crude Sample Compatibility. Proteins can be assayed in crude mixtures such as cell lysates or hybridoma supernatants, glycerol, or in up to 10% DMSO, which drastically reduces sample preparation.

Octet HTX System

Enables the highest throughput large molecule characterization as well as small molecule and fragment analysis. The system supports two 384- or 96-well microplates and provides up to 96-well simultaneous read-out, biosensor re-racking and automation compatibilities.

Octet RED384 System

Enables large molecule characterization as well as small molecule and fragment analysis. The system supports two 384- or 96-well microplates, provides 16-well simultaneous read-out, biosensor re-racking and automation compatibilities.

Octet RED96e System

Enables large molecule characterization and small molecule and fragment analysis. The system supports one 96-well plate, provides 8-well simultaneous read-out and biosensor re-racking. Enables assays to be performed over a wider temperature range of 15-40C and longer experimental time up to 12 hours with evaporation control.

Octet QK^e System

Enables large molecule characterization. The system supports one 96-well plate, provides 8-well simultaneous read-out and biosensor re-racking.

Octet K2 System

Enables large and small molecule analysis for low throughput users. The 2-channel system supports one 96-well plate with biosensor re-racking.

Life science research, drug discovery, and process development applications include:

Kinetic Mode Measurements — how specific, fast and tight are the interactions? Quantitation Mode Measurements — what is the sample concentration? Measure association rates — ka Human IgG quantitation for antibody titer determination or cell line optimization Measure dissociation rates — kd Mouse IgG quantitation for discovery Determine affinity constants — KD Custom quantitation assays for your proteins on Streptavidin biosensors Simple yes/no binding — epitope binning/mapping High senitivity assays for sub-ng/mL protein quantitation Relative binding rates — affinity ranking Simple Dip and Read™ direct binding assays in crude media Off-rate screening Anti-HIS tag biosensor for His-tagged protein quantitation

Octet System Video

Literature References

References to ForteBio's products in published literature.