For Determination of Antibody Concentration
ForteBio's Protein G biosensors provide a rapid and direct method for quantifying mammalian IgGs from buffer, media or other complex matrices. Protein G is pre-immobilized onto the biosensors, and binds to rodent and many other mammalian IgGs with higher affinity than Protein A, but does not bind to IgM, IgD or IgA. In combination with the Octet® system, Protein G biosensors can streamline bioprocessing applications by providing precise results with minimal sample handling and turnaround times as fast as two minutes. The biosensors can be regenerated multiple times, providing a cost-effective and time-saving assay format. Using Protein G Biosensors, the Octet System supports applications from hybridoma screening to media development and chromatography mass balance.
Key Features
- Direct detection of human, mouse and rat IgGs
- Regenerable and cost-effective format
- Obtain ELISA data in minutes
| Antibody
| Protein L
| Protein A
| Protein G
|
| Rat Total IgG |
s |
w |
m |
| Rat IgG1 |
s |
w |
m |
| Rat IgG2a |
s |
nb |
s |
| Rat IgG2b |
s |
nb |
w |
| Rat IgG2c |
s |
s |
s |
| Rabbit IgG |
w |
s |
s |
| Mouse IgG1 |
s |
w |
m |
| Mouse IgG2a |
s |
s |
s |
| Mouse IgG2b |
s |
s |
s |
| Mouse IgG3 |
s |
s |
s |
| Goat IgG |
nb |
w |
s |
| Sheep IgG |
nb |
w |
s |
| Bovine IgG |
nb |
w |
s |
| Guinea Pig IgG |
w |
s |
w |
| Hamster IgG |
s |
m |
m |
| Pig IgG |
s |
s |
w |
| Horse IgG |
— |
w |
s |
| Donkey IgG |
— |
m |
s |
| Dog IgG |
— |
s |
w |
| Cat IgG |
— |
s |
w |
| Human IgG1 |
s |
s |
s |
| Human IgG2 |
s |
s |
s |
| Human IgG3 |
s |
w |
s |
| Human IgG4 |
s |
s |
s |
s=strong m=medium w=weak nb=no binding |
|
Table 1: Proteins A, G and L possess unique affinity profiles toward antibodies from different species and isotypes. Protein G binds with high affinity to several more species and isotypes than Protein A.
For More Information
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